Applied Biosystems has developed a line of reagents for streamlining the process of quantitative reverse transcription PCR in RNA-based research applications. The High Capacity RNA-to-cDNA Master Mix and High Capacity RNA-to-cDNA Kit are for first-strand cDNA synthesis; TaqMan RNA-to-CT 2-Step Kit and Power SYBR Green RNA-to-CT 2-Step Kit are for screening a large number of targets from the same sample; and the TaqMan RNA-to-CT 1-Step Kit (to be released April 2008) and the Power SYBR Green RNA-to-CT 1-Step Kit are for one-step workflows with reduced hands-on processing.
In one-step reverse transcription quantitative PCR kits, all reactions from reverse transcription to quantitative PCR occur in a single tube, and are most effective for the detection of a single target from many samples or when a streamlined process with minimal chance of cross-contamination is desired. Two-step reverse transcription PCR kits offer flexibility when screening for multiple targets or archiving cDNA libraries.
www.appliedbiosystems.com/qrtpcrThis article was published in Drug Discovery & Development magazine: Vol. 11, No. 4, April, 2008, pp. 48.
