In vivo CCD-based imaging instruments are ideal for both rapid screening and in-depth in vivo analysis for fluorescent protein expressed in plants and animals. The figure illustrates testing of an anticancer agent with in vivo imaging. Bacteria, while typically thought of as an infectious agent, also has potential has an anticancer agent.1,2 A time-controlled sequence of fluorescent images was taken of two groups of mice. Both groups received an injection of RFP U87 brain cancer cells expressing red fluorescent protein (RFP). These cells produced the RFP fluorescent tumors shown. Comparing the untreated control group (A) to treatment (B) at day 14 indicates that the tumor was targeted by Salmonella A1-R given intravenously via weekly injections. This resulted in a significant reduction in tumor growth. The use of single- and multiple-color imaging of tumors is critical to time course studies and illustrates the importance of fluorescent protein imaging in cancer research. The iBox Scientia in vivo imaging system has an extended imaging range into the NIR with ultracooled, high quantum efficiency CCD-based cameras, which are used for NIR fluorescent dye and Qdot (Invitrogen) tags.
1. Kimura H, et al. Targeted therapy of spinal cord glioma with a genetically modified Salmonella typhimurium. Cell Prolif. 2010; 43(1):41-48.
2. Zhao M, et al. Monotherapy with a tumor-targeting mutant of Salmonella typhimurium cures orthotopic metastatic mouse models of human prostate cancer. Proc Natl Acad Sci. 2007;104(24):10170-10174.