Next-Gen Sequencing Sample Preparation
Beckman Coulter’s SPRIworks HT, a high-throughput solution for fragment library preparation on Illumina next generation sequencers, has a reagent kit for library preparation that includes sizing solution and PCR reagents. Protocols are automated from a suite of methods built on the Biomek FXP Liquid Handling Workstation. Built-in SPRI (Solid Phase Reversible Immobilization)-based size selection is included, along with a suite of methods that automate downstream processes such as PCR setup, PCR cleanup, qPCR setup, quantitation, normalization, and sample pooling.
In addition to library construction, the system offers per well size selection so application samples can be processed on the same plate. The system prepares 96 libraries and can be used with existing SPRIworks platforms, SPRI paramagnetic bead-based chemistry, and Biomek liquid handling platforms.