MicroCal introduced the latest advance in Isothermal Titration Calorimetery (ITC), the Auto-iTC200, which is designed to address the needs of today’s life science researchers – particularly those in drug discovery and development.
The system combines the performance of the ultra-sensitive iTC200 with full automation to run of up to 75 samples per day. The Auto-iTC200 enables the determination of all binding parameters in a single experiment, label-free and without the need for immobilization, using as little as five-10 ?g of protein per experiment. This system provides scientists with a powerful tool to develop new drugs without the limitations imposed by traditional assay systems, which often lack biological relevance, according to MicroCal.
The Auto-iTC200 is fully automated with all functions operated through software which includes integrated design wizards to assist in selecting experimental run parameters. Fully automated post-run data analysis utilizes Origin. Results are also exported in Microsoft Excel format for further analysis or data transfer. A wide range of applications can be accomplished with the Auto-iTC200 including: characterization of molecular interactions of small molecules, proteins, antibodies, nucleic acids, lipids and other biomolecules, enzyme kinetics, assessing the effects of molecular structure changes on binding mechanisms (SAR), and biotherapeutic construct selection.