High-Throughput Patch Clamp Systems
Fluxion Biosciences introduces the IonFlux-HT and IonFlux-16 Automated Patch Clamp Systems for ion channel research. These new systems combine Fluxion’s proprietary microfluidic technology with integrated electronics to automate the traditional patch clamp assay. Applications include primary/secondary screening, lead optimization, and toxicology screening.
Ion channels are ideal drug targets, but their potential has been largely untapped. Traditional manual approaches to patch clamp assays are time-consuming and skill-sensitive. Newer automated systems deliver a maximum of about 2,000 data points per day but the hardware costs are high. Consumable costs per data point have ranged from $1 to $4
The IonFlux-HT system includes 64 amplifiers and delivers between 8,000 and 10,000 assays per day at a cost of just 30 cents per data point using 384-well plates, according to the company. The IonFlux-16 provides all the features of the HT system with 16 amplifiers, utilizing a 96-well plate format. The IonFlux-16 system delivers 2,000 data points per day,
Both the IonFlux-HT and IonFlux-16 systems utilize standard well-plate formats with integrated microfluidic channels for cell patching and compound addition. The systems employ a familiar plate reader format for simplicity and integration with existing automated liquid handlers. The IonFlux technology automates cell manipulation, trapping, sealing, whole-cell formation, and precise voltage protocols. Voltage clamping occurs continuously during all fluidic operations. Automated compound addition during the voltage protocol provides delivery to the cells in 50 milliseconds, making the system ideal for ligand-gated channels.