The need for more complete and physiologically predictive cancer invasion models has driven the development of the 3D cell invasion assay (3500-096-K). There is growing evidence that tumor cell aggregates or spheroids are more representative of tumors in vivo, and they exhibit several physiological traits including similar morphology, the formation of cell-cell bonds, decreased proliferation rates, increased cell survival, tumor dormancy, and a hypoxic core. Applying this model to in a 3D culture invasion assay provides a more physiological approach for assessing tumor invasion and providing a visual component that can be quantitated through image analysis.
The Cultrex 3D cell invasion assay is a 3D culture qualified 96-well spheroid formation plate with a specialized spheroid formation ECM to drive aggregation and/or spheroid formation of cells. Upon completion of spheroid formation, the spheroid is embedded in an invasion matrix composed of basement membrane proteins. This matrix forms a hydrogel network through which invasive cells can travel. At this point, invasion modulating agents can be applied to the system to evaluate the impact on cell response. Cell invasion is visualized microscopically and can be quantitated through image analysis software.