Benzo(a)pyrene (BP) is a widespread environmental pollutants that has been found in cigarette smoke, charred food, exhaust from internal combustion engines and coal-burning factories. A highly reactive diol epoxide derivative of BP, (BPDE-I), is the major electrophilic, mutagenic, and carcinogenic metabolite of BP which is covalently bonded to the DNA and protein. The formed BPDE adduct, which is a biomarker of the body’s metabolic response to exposure, is associated with increased risk of lung and breast cancer. BPDE is also a frequently used biomarker of oxidative DNA damage and oxidative stress.
To address this need Trevigen offers a validated HT BPDE competitive ELISA kit (4360-096-K), a fast and sensitive (5 nM BPDE-HSA) immunoassay for the detection and quantitation of BPDE adduct in plasma, serum and cell culture supernatant samples. This assay employs a pre-coated BPDE-HSA monoclonal antibody, a labeled Biotinylated BPDE-HSA, a Strep-HRP conjugate, and chemiluminescent detection substrate to construct a high throughput assay flexible for your experimental design.