Gibson Assembly is a rapid and reliable method for the assembly of DNA fragments in a single-tube, isothermal reaction without reliance upon the presence of restriction sites within the target sequence. The technique is readily adaptable for site-directed mutagenesis.
Apoptosis, or programmed cell death, plays an essential role in organismal development and tissue homeostasis. During development, apoptosis is critical for the sculpting of organs and the elimination of unnecessary structures. Many cells die an altruistic death daily to secure homeostasis of the whole organism.
The most common metric used to assess tumor progression or response to treatment is the physical measurement of tumor length and width for the calculation of tumor volume. This approach is often hindered by the inherent inaccuracies and variability of hand calculation and is only useful for accessible subcutaneous tumors.
Since the isolation and propagation of the first immortalized cell line some 60 years ago, a multitude of relevant cell types and lineages now serve as a cornerstone in scientific research. However, as the “toolbox” grows so does our understanding of potential shortcomings in these models.
Electron microscopes are thousands of times more powerful than light microscopes, capable of visualizing single atoms and detecting differences in their positions as small as 50 picometers. Unfortunately, most biological specimens share characteristics that pose challenges to scientists’ ability to realize EM’s full potential.
Drug manufacturing processes―especially production of drugs delivered through inhalation―must have strict control over contaminate particles. Numerous methodologies allow companies to monitor these particles, the challenge is gathering the information quickly enough in order to be able to detect manufacturing problems.
Low-energy electrons interact much more strongly with the sample than the high-energy electrons from classical transmission electron microscopes. Electrons in the LVEM5 are strongly scattered by organic materials resulting in strong differentiation of features.
Laboratory automation within the pharmaceutical industry has been expanding rapidly over the past decade, providing higher productivity, precision, and quality.
Automated colony-picking instruments have been used for many years to image and select “good” bacterial colonies from an agar plate, pick the good colonies with a pin, then inoculate a microplate filled with media. The pins were then sterilized and the process was repeated.
As scientists developed fluorescent proteins for use in cellular assays, a need for a corresponding analytical instrument arose. The most important of these is the confocal microscope. Using various fluorescent proteins, real-time pictures and movies of cellular processes can be recorded using a confocal microscope.
Contamination is a significant concern for the pharmaceutical industry. Despite stringent gowning procedures and sophisticated PPE, human operators indisputably remain the greatest contributor to cleanroom and product contamination. For this reason, removing human operators from the equation is essential to reducing contamination.
With high demand for reliable and accurate data across the drug discovery industry, automation has become a key component within almost every laboratory. The ability to streamline workflows in an efficient manner increases walk-away time for lab personnel and frees them up for more-demanding tasks.
Cells naturally grow in a 3D environment, which has a direct influence on individual cell morphology and leads to the formation of complex intercellular structures. Maintaining this 3D environment is crucial to understanding cell function and signaling, as well as cellular responses to external stimuli such as pharmaceuticals and biopharmaceuticals.
While quantitative PCR is used for applications such as expression profiling, traditional end-point PCR is most commonly used to amplify DNA and RNA for downstream applications.
The growing demand for biomarker screening experiments has created a greater need for instruments that enable high-throughput capabilities coupled with high accuracy and low cost.
Large-scale, PCR-based gene analysis is only possible when signals are generated, captured, and analyzed accurately and reproducibly.
The Corning Epic platform offers access to native cell signaling pathways that best reflect relevant in vivo conditions, more accurately reflecting their true biological function.
Many companies are refocusing their drug discovery programs to provide a broader perspective of modes of action―as well as inclusion of biomarkers.
Across academic and drug discovery research there is growing demand for more physiologically-relevant assay platforms, leading to increased adoption of both label-free technologies and stem cells.
Surface plasmon resonance is used to detect the interaction of biomolecules in real time without the need for labels. Traditionally, SPR has been used to measure protein interactions during drug discovery research.
Though next-generation DNA sequencing provides very high levels of coverage even on complex genomes, it is still advantageous to reduce the complexity of samples.
Next-generation sequencing (NGS) has revolutionized genomics, enabling entire genomes and exomes to be sequenced more efficiently than ever before.
The Modular Automated Processing System from FEI is a workflow application that allows the microscopist to use image data from a light microscope to quickly and easily find and image the same area of the sample in an electron microscope.
Traditional compound microscopes offer high resolution, allowing visualization of structures down to approximately 220 nm, but at the cost of working distance and image field size.
With the availability of both mice and cancer cell lines that are genetically encoded to express fluorescent protein tags, tumor seeding, growth, and spread via metastasis can be viewed in real time in the living animal.
Atomic force microscopy is part of a broad class of scanning probe microscopes that were originally developed in the 1980s. AFMs physically track samples with a microfabricated probe to generate 3D topographical images.