Failure to repair biologic and environmental DNA damage, particularly double strand breaks (DSB), results in mutations and chromosomal aberrations that can lead to cellular dysfunction and death, genomic instability, and carcinogenesis. In this report we show ImageStream® methods for quantifying DSB foci using automated -H2AX and 53BP1 spot counting, both in tumor cell lines and in primary murine CD8+ T cells.
The ImageStream® imaging flow cytometry platform was used to study the mechanism of action of...
Many assays for immune function require imaging, but immune cells present significant challenges...
Increasing the number of samples which can be run simultaneously is desirable for high content...
In this report, apoptosis was induced in HL60 cells with daunorubicin, a DNA-intercalating agent which inhibits DNA and RNA synthesis and is used as a treatment for acute myeloid leukemia (AML). HL60 cells were exposed to a time course of daunorubucin treatment, and the apoptotic rate was measured directly from nuclear images obtained with the ImageStream system.
Purified proteins are required for many proteomics applications such as X-ray crystallography, NMR, mass spec, and in vitro biochemical assays. Proteins can be isolated from tissue or, more often, by their overexpression in an organism, like bacteria, yeast, or mammalian cells in culture.
Gold Nanorods (AuNR) hold great promise for biomedical imaging. AuNRs have very strong absorption peaks in the visible and near-infrared region due to a plasmonic effect; the aspect ratio of the AuNR directly determines the wavelength of the peak. For biomedical imaging, it is important to have optically pure samples of gold nanorods, which require physical purity as well.
This application note focuses on two challenging areas for nanoparticles: reliable, rapid scale-up of nanoparticle purification; and quantitative analysis of the concentration of species present.
Refractometers are used to continuously monitor the concentrations of liquids used in the food and beverage, chemical, energy, and metalworking industries, among others. Download this research study to learn how to your reduce costs by tightly controlling product yield.
Hypromellose (HPMC) capsules were originally formulated with a secondary gelling agent. This agent can delay dissolution in some circumstances and lead to unwanted issues during product development. In this article, we discuss the rationale for developing Capsugel’s Vcaps Plus capsules without a gelling agent.
We will examine the challenges of phenotypic screening, including the remaining technology gaps and underserved model systems.
By taking a holistic approach to lab services, the Efficiency-by-Design model systematically maps lab activities and needs to create a roadmap to increased productivity tailor-made to individual labs.
Nearly every day immunotherapy and immune-oncology make headlines in both the pharmaceutical industry and mainstream news. But what exactly is immunotherapy and why is so much attention being focused on it?
The Gram-negative bacterium E. coli is the workhorse of molecular biology, regularly used as the first choice host for DNA cloning, small-scale protein expression, and large-scale protein production for FDA approved biologics.
Purifying low level compounds, such as those typically isolated from natural products, is an area of growing interest for the purpose of discovering new chemical entities.
The ACQUITY UPLC I-Class System was optimized to reduce extra-column band spread in each of the contributing components post-injection. Newly designed UV Detector flow cells use the same optical path as their ACQUITY UPLC predecessors so the same high sensitivity is achieved; however, fluidic lines and connections have been re-designed to further reduce band spread.
The Accuri C6 Flow Cytometer® simplifies cell analysis by performing absolute cell counts, eliminating the need for counting beads, or dual-platform hemacytometer counts. The microprocessor-controlled peristaltic pump system allows the sample volume pulled per run to be determined.
Selection of the appropriate ionization mode for a given substance is relatively easy if the properties of the substance are known beforehand. When this is not the case, the time required for selecting the best ionization mode for each compound can have a negative impact on R&D efficiency.
Robots have long played a central role in drug discovery and testing. In order to choose a robot that will be of the greatest benefit, those involved in the selection process should be familiar with basic robot concepts. This paper presents key relevant aspects of robot technology and includes a section, "10 Things to Consider When Choosing a Robot."