Failure to repair biologic and environmental DNA damage, particularly double strand breaks (DSB), results in mutations and chromosomal aberrations that can lead to cellular dysfunction and death, genomic instability, and carcinogenesis. In this report we show ImageStream® methods for quantifying DSB foci using automated -H2AX and 53BP1 spot counting, both in tumor cell lines and in primary murine CD8+ T cells.
The ImageStream® imaging flow cytometry platform was used to study the mechanism of action of...
Many assays for immune function require imaging, but immune cells present significant challenges...
Increasing the number of samples which can be run simultaneously is desirable for high content...
This white paper explores ways to maximize multi-material and color possibilities utilizing 3D printers with the capability to print ready to use models, like the Connex3 Objet500. 3D printing with the ability to use multiple materials and a variety of color choices results in enhanced functionality and product realism that brings parts to life. This White Paper will Cover: Flexible and Ridged Pallets Workflows Color Ranges Workflows Digital Models
The epMotion® 5075 TMX is a clean liquid handling system that provides a robust, reproducible and reliable method for obtaining genomic DNA from biological samples suitable for real-time qPCR and STR profiling. The epMotion 5075 TMX system can run a maximum of 96 samples in approximately 2.5 hours significantly minimizing hands-on time spent on manual extraction. This allows for timely processing of case-work samples and can cut down on the back-log of samples found in most crime laboratories.
Gas chromatographs (GC) are connected to one or more cylinders of high purity carrier gas. To achieve better results of their analysis at lower and lower concentration levels, instrument operators opt for ultra high purity gas of 99.999% or better. But just buying the best gas does not solve the problem; you must be able to transfer the gas from the cylinder to the instrument without contaminating the gas. The design of the transfer system is critical.
Cardiovascular disease is the leading cause of mortality in the United States. As early diagnosis is critical, interest in measurement of CVD biomarkers has increased dramatically. CVD biomarker discovery research has focused on arterial plaque related disease as conditions are prevalent, chronic and progressive and can be measured by soluble biomarkers.
Drug-induced liver injury (DILI) is an adverse event that results in withdrawal of drugs from market. As drugs may cause liver injury through various mechanisms, measuring multiple biomarkers is required to determine the potential for hepatotoxicity in early drug development.
Sterility testing of final drug products is a mandatory release test. Traditional methods such as membrane filtration require a minimum incubation time of 14 days. This extended time to result delays product release and increases storage costs. Download this white paper and learn how to achieve results in just 5 days!
Dysregulation of angiogenesis, a fundamental process in growth, development and tissue repair, leads to abnormal blood vessel growth involved in many common diseases and plays a significant role in cancer tumor growth and metastasis. We demonstrate the utility of the MILLIPLEX® MAP Human Angiogenesis/Growth Factor Panel , based on the Luminex® xMAP® bead-based multiplexed assay platform.
Mitochondria are unique and complex organelles that perform essential functions in many aspects of cell biology. The dominant function of mitochondria is the production of more than 90% of the cell’s energy in the form of ATP through oxidative phosphorylation.
Multiple sclerosis is a common cause of neurologic disability in young adults. Based on the past course of MS disease, several patterns of progression or subtypes have been described. In recent years, growing evidence suggests the value of peripheral biomarkers in the diagnosis and prognosis of CNS disorders with the hope to improve clinical outcomes.
Testing for microbial contamination in complex sample matrices is critical to monitor product quality and ensure compliance with regulatory requirements and product safety. Contamination is usually assessed by membrane filtration and incubation on solid agar media with results not available for several days.
Membrane filters with a 0.45 μm pore size have long been recognized as the standard for growth of microorganisms. However, there is little published literature comparing the effects of different pore sizes on colony size and recovery. This whitepaper examines the influence of different pore sizes on some common microorganisms.
This whitepaper describes use of the Milliflex® Quantum system for accelerated detection of contamination in water samples. The study assessed the ability of the system to detect microbial contaminants quicker and to evaluate the reduction of time-to-final result in comparison with the compendial method.
The EZ-Fit™ Filtration Unit is a disposable filtration device for bioburden testing of liquid samples including water, raw materials, in-process samples, and final products. It is designed to ensure optimal microbial recovery and brings a new standard for ease of use.
In this report, apoptosis was induced in HL60 cells with daunorubicin, a DNA-intercalating agent which inhibits DNA and RNA synthesis and is used as a treatment for acute myeloid leukemia (AML). HL60 cells were exposed to a time course of daunorubucin treatment, and the apoptotic rate was measured directly from nuclear images obtained with the ImageStream system.
Purified proteins are required for many proteomics applications such as X-ray crystallography, NMR, mass spec, and in vitro biochemical assays. Proteins can be isolated from tissue or, more often, by their overexpression in an organism, like bacteria, yeast, or mammalian cells in culture.