Failure to repair biologic and environmental DNA damage, particularly double strand breaks (DSB), results in mutations and chromosomal aberrations that can lead to cellular dysfunction and death, genomic instability, and carcinogenesis. In this report we show ImageStream® methods for quantifying DSB foci using automated -H2AX and 53BP1 spot counting, both in tumor cell lines and in primary murine CD8+ T cells.
The ImageStream® imaging flow cytometry platform was used to study the mechanism of action of...
Many assays for immune function require imaging, but immune cells present significant challenges...
Increasing the number of samples which can be run simultaneously is desirable for high content...
No matter how routine the analysis, care must be taken when preparing fresh titrants and standardizing them; calibrating and maintaining appropriate sensors and handling samples. This guide discusses critical factors that can affect titration results and provides insight into how to elimination some of the more common sources of titration errors.
A new breed of manual pipettes has emerged that, unlike traditional pipettes which utilized a single rotating plunger to set volumes, features a revolutionary approach to manual pipetting. This new class of manual pipettes utilize an adjustable dial system to help reduce repetitive stress injuries and allow the user to set volumes ten times faster. Coupled with unique plunger settings that permit customized plunger force, this new evolution in manual pipettes improves manual pipetting techniques tenfold.
As reaction components for real-time PCR experiments come in a range of different labware formats, scientists traditionally have used single channel pipettes to prepare the reaction mixes. Unfortunately preparing and transferring samples one by one is not only error prone but also very time consuming. In this technical note the authors compare the results from using an 8-channel VOYAGER pipette and a conventional single channel pipette to prepare eight samples of extracted human gDNA in twelve replicates for real-time PCR.
Many assays for immune function require imaging, but immune cells present significant challenges to image-based analysis due to their rarity and the need for simultaneous multispectral immunophenotyping, making statistically robust quantification difficult. Thus, immune function assays should ideally be performed by an imaging flow cytometry platform that has the ability to quantify the imagery of a large populations of cells.
The production of pharmaceutical products requires the analysis of a series of essential trace elements as well as elements that are toxic in larger concentrations. Aspects such as bio-availability, toxicity and quantity of the elements must be considered. The sources of such impurities can be production-related contamination from sieving and grinding processes, treatment with catalysts, and transportation in piping.
Are you annoyed with inaccurate pipetting results? With these 10 simple tips, you will improve your results instantly! Learn: What to do before you start pipetting How to pipette How to optimize pipetting performance
In this article we focus on a selection of good pipetting techniques, which if maintained, offer the best chance of increasing and maintaining the reproducibility of your serial dilutions. Mixing is a key component of dilution protocols and in this article we additionally discuss different mixing parameters to help you achieve a homogenous sample.
In this Application note, we highlight the development of an LC method that optimized the Chromatographic resolution for the released N-glycans that are commonly found in mAbs, including the high mannos N-glycan structures that are known to negatively affect circulation half-life as well as indicate aberrant cell culture conditions.
This white paper explores ways to maximize multi-material and color possibilities utilizing 3D printers with the capability to print ready to use models, like the Connex3 Objet500. 3D printing with the ability to use multiple materials and a variety of color choices results in enhanced functionality and product realism that brings parts to life. This White Paper will Cover: Flexible and Ridged Pallets Workflows Color Ranges Workflows Digital Models
The epMotion® 5075 TMX is a clean liquid handling system that provides a robust, reproducible and reliable method for obtaining genomic DNA from biological samples suitable for real-time qPCR and STR profiling. The epMotion 5075 TMX system can run a maximum of 96 samples in approximately 2.5 hours significantly minimizing hands-on time spent on manual extraction. This allows for timely processing of case-work samples and can cut down on the back-log of samples found in most crime laboratories.
Gas chromatographs (GC) are connected to one or more cylinders of high purity carrier gas. To achieve better results of their analysis at lower and lower concentration levels, instrument operators opt for ultra high purity gas of 99.999% or better. But just buying the best gas does not solve the problem; you must be able to transfer the gas from the cylinder to the instrument without contaminating the gas. The design of the transfer system is critical.
Cardiovascular disease is the leading cause of mortality in the United States. As early diagnosis is critical, interest in measurement of CVD biomarkers has increased dramatically. CVD biomarker discovery research has focused on arterial plaque related disease as conditions are prevalent, chronic and progressive and can be measured by soluble biomarkers.
Drug-induced liver injury (DILI) is an adverse event that results in withdrawal of drugs from market. As drugs may cause liver injury through various mechanisms, measuring multiple biomarkers is required to determine the potential for hepatotoxicity in early drug development.
Sterility testing of final drug products is a mandatory release test. Traditional methods such as membrane filtration require a minimum incubation time of 14 days. This extended time to result delays product release and increases storage costs. Download this white paper and learn how to achieve results in just 5 days!
Dysregulation of angiogenesis, a fundamental process in growth, development and tissue repair, leads to abnormal blood vessel growth involved in many common diseases and plays a significant role in cancer tumor growth and metastasis. We demonstrate the utility of the MILLIPLEX® MAP Human Angiogenesis/Growth Factor Panel , based on the Luminex® xMAP® bead-based multiplexed assay platform.